Genomic functional analysis of cancer cells by interfering RNA for the identification of regulatory networks associated with proliferation and death in response to genotoxic chemotherapy.

The aim of this project was to identify the regulatory networks of the cancer cell proliferation phenotype after chemotherapy, as a model of cell proliferation after DNA damage. For this, techniques were used that allowed the resolution at the level of the subpopulations of interest. Biocomputational tools were generated to allow such analysis, as well as a model for the analysis of the data and the generation of hypotheses of potential control points of the phenomenon, which were validated experimentally.

This was intended to generate the basic mechanistic knowledge of regulation of a phenomenon induced by a variety of physical and chemical agents of relevance in health. After DNA damage, some cells are able to proliferate in the presence of such damage. The proliferation of cells with damage to their DNA can favor a malignant transformation of non-tumor cells exposed to these agents, and could play a role in the development of other pathologies. Additionally, this proliferation in the presence of damage could favor an evolution towards more aggressive and multiresistant phenotypes in cancer cells treated with chemo or radiotherapy.